Complete kit-the SMARTer RACE 5вЂ™/3вЂ™ Kit contains all the necessary components (save gene-specific primers) to streamline your process.

Easy to use-the SMARTer RACE protocol is performed in a single tube, and minimizes handling of both your RNA sample and your synthesized cDNA.

The SMARTer RACE 5'/3' procedure combines SMART first-strand cDNA synthesis technology with a powerful suppression PCR protocol that vastly reduces the background amplification commonly associated with RACE protocols.

The kit includes our highly robust SeqAmp DNA Polymerase for amplification of products, and the In-Fusion HD Cloning Kit for efficient cloning of RACE fragments in the provided linearized vector. The new kit is also designed to accommodate larger RNA input volumes (up to 11 Вµl) and has been shown to perform more efficiently on challenging targets (e.g., those that are long, GC-rich, etc.). The SMARTer protocol does not involve any of the adaptor ligation steps that other RACE kits incorporate, making the protocol shorter and significantly easier to execute. First-strand cDNA generated with this kit can be used directly in 5'- and 3'-RACE PCR reactions. The SMARTer RACE 5'/3' Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. The SMARTer RACE 5'/3' Kit is an improved version of the SMARTer RACE cDNA Amplification Kit.

RACE can provide the sequence of an RNA transcript from a short, known sequence within the transcript all the way to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. Rapid amplification of cDNA ends (RACE) is a technique used to obtain the full-length sequences of RNA transcripts.

Gene construction kit